Professor Steven L. Jacques
Steven L. Jacques
Professor, Biomedical Engineering & Dermatology
Oregon Health & Science University CH13B 3303 SW Bond Avenue, Portland, OR 97239, USA
The basics of tissue optics will be presented. The origins of optical properties in tissues are discussed, linking the biological structures encountered in cells and tissues to the optical properties that govern light transport. Imaging and spectroscopic sensing based on absorption, fluorescence, Raman scattering and elastic light scattering are introduced. The properties of absorption coefficient, scattering coefficient, anisotropy of scattering, and refractive index are discussed. The use of these properties in optical transport theory, including Monte Carlo simulations, diffusion theory, and the Pn method, will be demonstrated. Examples of common optical measurements demonstrate how measured observations are interpreted by theory to specify the tissue optical properties. The influence of optical properties on optical contrast based on absorption, fluorescence or scattering is discussed. The ability to measure optical properties and use these properties in theory to predict optical behavior in experimental situations is a basic skill needed in biomedical optics and biophotonics.
S. Jacques, and B. Pogue, "Tutorial on diffuse light transport," J. Biomed. Opt. 13, 041302 (2008).
(download and read Sections 2.1-2.3)
S. L. Jacques, "Optical properties of biological tissues: a review," Phys. Med. Biol. 58, R37–R61 (2013).