Biophotonics '09: Lecture by Prof. Darryl Bornhop

Interferometry for measuring molecular interactions

Professor Darryl J Bornhop

Department of Chemistry
Vanderbilt University
VU Station B 351822
Nashville, TN 37235-1822, USA

Tel: +1 615 322 4226 {Direct}
Toll free: (800) 288-5400 {US only}
Fax: +1 615 343 1234

E-mail: darryl.bornhop@vanderbilt.edu
Web: http://www.vanderbilt.edu/AnS/Chemistry/groups/bornhop/

Abstract

Building on decade of experience, we recently demonstrated that Back-scattering Interferometry (BSI) could be used to perform label-free molecular interaction determination over six decades in binding affinity (mM-pM), at physiologically relevant concentrations, with nanoliters of sample and in free solution [1].   We will discuss BSI and several other relevant interferometric techniques (Mach-Zehnder (or Young), Porous Si, Dual Polarization and Diffraction Optics-based, BioCD [2-4]), in the context of their strengths and weaknesses for label-free interaction studies ranging from drug screening to point-of-care or near patient diagnosis.   

References

[1] D. J. Bornhop, J. C. Latham, A. Kussrow, D. A. Markov, R. D. Jones, and H. S. Sorensen, "Free-solution, label-free molecular interactions studied by back-scattering interferometry," Science 317, 1732-1736 (2007).

[2] Juan Wang, Xiaowen Xu, Zhanxia Zhang, Fan Yang, and Xiurong Yang, "Real-Time Study of Genomic DNA Structural Changes upon Interaction with Small Molecules Using Dual-Polarization Interferometry," Anal. Chem., article published on Web 14 May 2009 (downloaded from http://pubs.acs.org).

[3] M. M. Varma, D. D. Nolte, H. D. Inerowicz, and F. E. Regnier, "Spinning-disk self-referencing interferometry of antigen-antibody recognition," Opt. Lett. 29, 950-952 (2004).

[4] Leo L. Chan, Maria Pineda, James T. Heeres, Paul J. Hergenrother and Brian T. Cunningham, A General Method for Discovering Inhibitors of Protein−DNA Interactions Using Photonic Crystal Biosensors, ACS Chem. Biol. 3(7), 437–448 (2008).

Additional reading

[5] K. P. S. Dancil, D. P. Greiner, and M. J. Sailor, "A porous silicon optical biosensor: Detection of reversible binding of IgG to a protein A-modified surface," Journal of the American Chemical Society 121, 7925-7930 (1999).

[6] J. B. Goh, R. W. Loo, and M. C. Goh, "Label-free monitoring of multiple biomolecular binding interactions in real-time with diffraction-based sensing," Sensors and Actuators B-Chemical 106, 243-248 (2005).

Slides

• Introduction
• Interferometry for measuring molecular interactions

Link to program

• Session 10

Return